Sci. Pharm. 2011; 79: 673–694
Amoxicillin Loaded Chitosan–Alginate Polyelectrolyte Complex Nanoparticles as Mucopenetrating Delivery System for H. Pylori
Saahil ARORA *, Sankalp GUPTA, Raj K. NARANG, Ramji D. BUDHIRAJA
Nanomedicine Research Centre, Department of Pharmaceutics, I.S.F. College of Pharmacy, Moga-142001 (Punjab), India.
* Corresponding author. E-mail: email@example.com (S. Arora)
The present study has been undertaken to apply the concept of nanoparticulate mucopenetrating drug delivery system for complete eradication of Helicobacter pylori (H. pylori), colonised deep into the gastric mucosal lining. Most of the existing drug delivery systems have failed on account of either improper mucoadhesion or mucopenetration and no dosage form with dual activity of adhesion and penetration has been designed till date for treating H. pylori induced disorders. In the present study, novel chitosan-alginate polyelectrolyte complex (CS-ALG PEC) nanoparticles of amoxicillin have been designed and optimized for various variables such as pH and mixing ratio of polymers, concentrations of polymers, drug and surfactant, using 33 Box-Behnken design. Various studies like particle size, surface charge, percent drug entrapment, in-vitro mucoadhesion and in-vivo mucopenetration of nanoparticles on rat models were conducted. The optimised FITC labelled CS-ALG PEC nanoparticles have shown comparative low in-vitro mucoadhesion with respect to plain chitosan nanoparticles, but excellent mucopenetration and localization as observed with increased fluorescence in gastric mucosa continuously over 6 hours, which clinically can help in eradication of H. pylori.
Factorial design • Mucopenetration • Helicobacter pylori • Box Behnken design • Stomach specific delivery system
Received November 15th, 2010 | Accepted May 18th, 2011 | Published Online May 19th, 2011