Sci Pharm; 2009; 77: 827–835
An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew (Drosera rotundifolia L.)
Christoph WAWROSCH *, Eva BENDA, Brigitte KOPP
Department of Pharmacognosy, University of Vienna, Althanstr. 14, A-1090, Vienna, Austria
* Corresponding author. E-mail: christoph.wawrosch@univie.ac.at (C. Wawrosch)
Abstract
An efficient procedure for in vitro shoot production of the medicinal plant sundew (Drosera rotundifolia L.) was developed. Of three cytokinins tested, zeatin (ZEA) at a concentration of 2 μM resulted in the formation of large numbers of adventitious shoots on leaf explants. A larger size of the small shoots was achieved by combining a 2-weeks preculture with ZEA (step 1: shoot induction) with a 5-weeks main culture without plant growth regulators (step 2: shoot elongation). Liquid media were superior to semisolid media: An average of 27.4 shoots per leaf explant, and 53.3 shoots per shoot explant were achieved. The 2-step culture system with liquid media permits a comparatively cheap micropropagation of sundew as well as in vitro biomass production, with potential for scale-up.
Keywords
Endangered • Medicinal Plant • Liquid Medium • Micropropagation
Received August 4th, 2009 | Accepted September 21st, 2009 | Published Online September 21st, 2009
