Sci. Pharm. 2009; 77: 567–578
Preparation and Biological Evaluation of a [55Co]-2-Acetylpyridine Thiosemicarbazone
Amir R. JALILIAN *, Pejman ROWSHANFARZAD, Mehdi AKHLAGHI, Mahsheed SABET, Mohsen KAMALI-DEHGHAN, Mehrban POULADI
Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS-NSTRI), P.O.Box: 31485-498, Karaj, Iran
* Corresponding author. E-mail: ajalilian@nrcam.org (A. R. Jalilian).
Abstract
55Co](III)[APTS]2) was investigated. Co-55 (T1/2=17.53 h) was produced by 150 μA irradiation of a natural nickel target by 15 MeV protons. The 55Co was separated from the irradiated target material using a two-step method with a radiochemical yield of >95% followed by radionuclidic and chemical purity control. [55Co](III)chloride was mixed with 2-acetylpyridine thiosemicarbazone for 30 min at room temperature to yield [55Co](III)[APTS]2 (radiochemical purity > 98% shown by RTLC/HPLC). A specific activity of about 10–20 Ci/mmol was obtained. The final solution was diluted in normal saline to 5% ethanolic solution for biological evaluation. The stability of the final product was checked in the absence and presence of human serum at 37°C to 24 h. The partition co-efficient of the final complex at the pH of 7 was 1.00±0.08. A significant tumor accumulation (%ID/g; 3.5%) was observed in tumoral tissue 21 h post injection in fibrosarcoma-bearing mice by biodistribution studies. Co-incidence imaging also demonstrated tumor uptake from 21–35 h however at 35 h tumor uptake is more specific and significant.
Keywords
Cobalt-55 • Thiosemicarbazone • Biodistribution • Tumor imaging • Co-incidence • Fibrosarcoma
Received July 4th, 2009 | Accepted August 3rd, 2009 | Published Online August 5th, 2009
