Sci. Pharm. 2009; 77: 605–616
HPLC Analysis and Standardization of Arjunarishta – An Ayurvedic Cardioprotective Formulation
Uma R. LAL, Shailendra M. TRIPATHI, Sanjay M. JACHAK, Kamlesh K. BHUTANI, Inder P. SINGH *
Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. A. S. Nagar 160062, Punjab, India
* Corresponding author. E-mails: ipsingh@niper.ac.in or ipsingh67@yahoo.com (I. P. Singh).
Abstract
Arjunarishta (Parthadyarishta) is an important Ayurvedic formulation used for cardiovascular disorders and is prepared by fermenting the decoction of specified plant materials using flowers of Woodfordia fruticosa. In present communication, an HPLC-PDA method was developed for the standardization of Arjunarishta by quantitative estimation of major antioxidant compounds, ellagic acid, gallic acid, ethyl gallate, quercetin and kaempferol as markers. The developed method was validated with respect to linearity, precision, accuracy, and robustness. The HPLC analysis showed an increase in amount of ellagic acid and gallic acid during preparation, i.e. decoction vs formulation. A similar increase in free radical scavenging activity of formulation vs decoction was also observed. Arjunolic acid and arjunic acid were not detected in the formulation.
Keywords
Parthadyarishta • Gallic acid • Ethyl gallate • Ellagitannins • Flavonoids
Received June 1st, 2009 | Accepted July 29th, 2009 | Published Online July 30th, 2009
