Sci. Pharm. 2009; 77: 343–354
Evaluation of a [67Ga]-Thiosemicarbazone Complex as Tumor Imaging Agent
Amir R. Jalilian * 1, Pegah Mehdipour 2, Mehdi Akhlaghi 1, Hassan Yousefnia 1, Kamaledin Shafaii 1
1 Radiolabeling Lab, Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS), Moazzen Blvd., Rajaeeshahr, P.O.Box: 31485-498, Karaj, Iran.
2 Amala Faculty of Pharmacy, Islamic Azad University, Tehran, Iran.
* Corresponding author. E-mail: ajalilian@nrcam.org (A. R. Jalilian)
Abstract
[67Ga]labeled 2-acetylpyridine 4,4-dimethylthiosemicarbazone ([67Ga]-[APTSM2]2+) was prepared using freshly prepared [67Ga]GaCl3 and 2-acetylpyridine 4,4-dimethylthiosemicarbazone (APTSM2) for 30 min at 90°C (radiochemical purity: >97% ITLC, >98% HPLC, specific activity: 15–20 Ci/mmol). Stability of the complex was checked in human serum for 37ºC. The biodistribution of the labeled compound in vital organs of normal and fibrosarcoma bearing mice were compared with that of free Ga3+ cation up to 24h. Initial SPECT images and biodistribution results showed significant tumor uptake in fibrosarcoma-bearing mice after 2 hour post injection.
Keywords
67Ga-complexation • Thiosemicarbazone • Biodistribution • SPECT • Fibrosarcoma
Received December 15th, 2008 | Accepted March 5th, 2009 | Published Online March 6th, 2009
